This investigation was carried out to study the effect of some natural organic nutrients including compost extract which recently called in agriculture field )compost tea( and chicken manure extract with different concentrations on yield, cluster characteristics, total sugars and nitrate content in berries of Thompson seedless grapevines. Seasonal changes of NPK in leaf petioles during different stages of grapevines were also studied. Treatment No. 8 (Compost tea (1:10) x chicken manure extract (1:10) gave the highest values of yield (kg/vine), cluster weight (g) and volume (ml) and also for total sugars and nitrate content of berries. From the other side, the best treatment for producing safety fruits for using human kind was the treatment No. 9 {(Compost tea (1:20) x chicken manure extract (1:20)}, which gave satisfactory yield and cluster weight and volume as well as total sugars (significantly more than values of control treatment), besides it produce the lowest nitrate contents of berries, that means its more safety for human use.