This investigation  was carried out during two successive seasons of 2004 and 2005 to  study the effect of fertilization by using two types of organic manures, i.e. cattle or town refuse at different levels (50, 75 and 100%) from its recommended dose with or without biofertilizers mixture, i.e. microbien and phosphoren in combination with 50% mineralized NPK on growth, chemical composition as well as yield and its quality of Grand Nain banana plants in comparison to banana plants only fertilized with mineralized 100% NPK of the recommended dose. The best results obtained  that from banana plants inoculated and fertilized with cattle manure at rate of 100% . In addition, biofertilizers with organic manure improved growth, chemical composition as well as fruit yield and its quality. Increasing the rate of either organic manure with or without inoculation had a positive increment on all studied parameters, especially with the high rate.  Chemical constituents of Grand Nain banana fruits were greatly improved as a result of using N in both organic and mineral sources rather than adding N completely via only mineral source. Organic fertilization with cattle or town refuse had a favorable effects on  fruit quality  since in increase finger weight and concentration of  soluble solids and  total sugar as well as  decreasing concentration of starch and nitrate in fruits except the high rate of town refuse. Organic fertilization by using cattle manure at the rate of 100% combined with 50% mineralized  NPK of the recommended rate and inoculated by biofertilizers gave the best results with all studied characteristics of Grand Nain banana plants .