This investigation was carried out at Horticulture Research Institute Orchard. AgricultureResearchCenter during the two successive seasons of 2005/2006 and 2006/2007 to study the effect of different treatments on germination and seedling growth of Annona sequamosa L. (Balady spp.) The treatments used were soaking in water, GA₃, fungicide and EM. The obtained results showed that, EM treatments increased germination percentage close to GA3 treatments and fasted rate of germination as compared with untreated treatment (control). Moreover, EM treatments increased stem length and diameter, number of leaves/plant, leaf area and root length, as well as stem, leaf and root fresh and dry weight. Leaf chlorophyll content insignificantly differed between EM and GA₃ treatments. Diluted EM treatment was more effective than concentrated one in this concern. Anatomical study of  Annona sequamosa seed showed that, embryo of fresh extracted seed was not yet complete differentiated and it fully developed when seed stored for long time, so germination of fresh seeds were uneven and irregular. In addition, morphological structure of seed show that water can be enters to seed through hillum.