Two field experiments were conducted at two locations; New Valley and Giza Agric. Res. Stations, ARC during the two successive summer seasons of 2007 and 2008 to study the influences of inoculation with Cyanobacteria, Azospirillum and Pseudomonas individually or in combination on forage yield and quality traits of teosinte ( local variety). The experimental design was a randomized complete block with three replications. Combined data over the two seasons at two locations indicated that biofertilizers inoculation significantly increased fresh and dry yields, plant height, crude protein, crude fiber, ash%, ether extract and nitrogen free extract and total digestible nutrient comparing to uninoculated plants through three cuts. Also, data confermed the superiority of inculation with mixture of inoculants in achieving higher values of all forage yield teosinte characters. Teosinte plants inoculated with Cyanobacteria, Azospirillum and Pseudomonas gave significant increases in fresh forage yield by about 149 and 140% as compared with the uninoculated plants receiving no nitrogen fertilizer at NewValley and Giza location, respectively.                 It is concluded that teosinte inoculated with biofertilizers in dual or in mixture and received the half dose of nitrogen fertilizer gave results near or similar those obtained from uninoculated teosinte and received the recommended dose (120kgNfed^-1) of nitrogen fertilizer.