A field experiment was conducted at privet farm of Demo (29° 17ˋ N, 30° 53ˋ E), Fayoum Governorate, Egypt, during 2018/2019 and 2019/2020 to study the effect of three humic acid treatments, i.e. (0 without humic acid as control, 3.5 and 7 Kg humic acid/fed) on quality and yield traits of eight sugar beet varieties (Santolhne, Pepite, Amina, Beta 401, Dina, Grinta, Sirona and Bts 302). Results indicated that root yield (ton/fed) was increased by increasing the rates of humic acid from 3.5 to 7kg per fed in the first season (27.02 and 29.11 ton/fed, respectively), corresponding (28.08 and 29.72 ton/fed in the second season respectively). Similarly, the content of molasses forming substances showed more reduction in 3.5 than in 7kg/fed treatment. Nevertheless, the trend of increasing content of sucrose and refined sugar was associated with humic acid rates. Results showed that varieties significantly differed among them where, Sirona variety show the superiority over the other seven tested varieties and recorded the highest values of root diameter (14.19 and 15.14 cm, respectively), fresh root weights/plant (1.41 and 1.47 kg, respectively), in both seasons, as well as root yield/fed (29.94) in 2^nd season. While, in first season variety (Bts 302) registered the highest values of root yield (29.42 ton/fed), also this variety recorded the highest sugar yield (4.62 and 5.23 ton/fed, respectively) in both seasons. Highly significant interaction effects between humic acid and varieties root yield and sugar yield and their related traits in both seasons. it could be concluded that fertilization with humic acid at a rate of 7kg /fed as a soil application to get the maximum of root and sugar yields/fed in sandy soil.