A greenhouse experiment was conducted to study the effect of different saponin sources on incidence of cotton seedling damping-off caused by Rhizoctonia solani, sclerotium rolfsii, Macrophomina phaseolina, Fusarium moniliforme, two isolates of Fusarium oxysporum and the mixture of these fungi on cotton cultivar Giza 86.  The experiment conducted also on flax cultivar Giza 8 by using the mixture of the texted fungi.  The sources of saponin were seeds of Artiplex nummularia, seeds of Leucaena leucocephala, roots of Medicavo sativa, and seeds of Luffa aegyptiaca.  Three concentrations (3, 6, and 9g or ml/kg seeds) of each saponin source were tested.  The fungicide Monceren at rate 3g/kg seeds was used to compare the efficiency of different sources of saponin.  The saponin sources at different concentrations and Monceren were used as seed dressing.  Efficiency of saponin in controlling damping-off disease differed depending on source of saponin, its concentration, the tested fungi, the tested isolate, and the host plant.  Cluster analysis was used to study the antifungal patterns of the saponin sources and to compare between the tested fungi in their response patterns to the different sources of saponin.