Two field experiments were carried out at Baramoon Experimental Station, Dakahlia Governorate, Egypt, during the two successive seasons of 2006/2007 and 2007/2008 to study the marjoram (Majorana hortensis Mnch) potential to survive under salinity condition and to find out the effect of some bio-fertilizers on this potential. The present investigation were planed to test (4) levels (0, 2000, 4000 and 6000 ppm) of saline water irrigation, as well as foliar nutrition of some bio-compounds i.e., (EM), Novatrein and Askobein as mixtures and their interactions on plant growth, essential oil, quantity and quality of marjoram. Marjoram plants showed a quite tolerance to salinity at 2000 ppm, whereas the excess concentrations( 4000 and 6000 ppm) caused a severe damage to the irrigated plants. A remarked reduction in fresh and dry weights of plant, survival percentage, essential oil percentage  and its components under different salinity concentrations. Gas Liquid Chromatography showed that saline water irrigation at levels of 2000 and 4000 ppm slightly decreased the percentages of main components whereas 6000 ppm caused vanishing of some constituents and caused severe harmful to oil principals. All bio-compounds i.e., (EM) and both of Novatrein or Askobein, slightly caused significant increments in the plant growth characters and displayed the best estimates in the two seasons. Application of these compounds raised the plant potential to tolerate salinity and  simulation  increased the survival percentage. In addition, utilizing these compounds slightly increased oil active principals and could overcome the harmful effect of saline irrigation water.