This study was carried out at El-Sheikh Zewaied Research Station, Desert Research Centre at North Sinai Governorate during the two seasons of 2003/ 2004 and 2004/ 2005 to study the effect of different levels of compost (0, 4, 8 and 12 m³/ fad.), saline water irrigation (600, 1500 and 3000 ppm) and their interaction on growth, active ingredients and chemical constituents of tree tobacco (Nicotiana glauca) plants. Results indicated that the maximum yield of active ingredients (total alkaloids, anabasine and rutin) and the highest values of growth characters (plant height, number of branches, dry weight of plant and dry weight of leaves/ plant) and plant minerals content (N, P, K, Zn, Mn and Fe) could be obtained when plants were irrigated with saline water at 1500 ppm with the addition of 12 m³/fad compost during soil preparation.