This study was carried out during the two seasons of 2003/ 2004 and 2004/ 2005 at the Experimental Station of Desert Research Center at El-Sheikh Zewaied, North Sinai Governorate; aiming to investigate the effect of different irrigation water rates (4, 8 and 12 L/ plant/ week), antitranspirants (6% CaCO₃, 5% Folicote and 10% CaO) as well as their interactions on plant growth, active ingredients, total carbohydrates and chemical constituents of Nicotiana glauca plants. The  obtained results showed that, the highest values of vegetative growth parameters (plant height, number of branches/ plant and dry  weights of plant and leaves/ plant), active ingredient (total alkaloids, anabasine and rutin percentages and contents/ plant), total carbohydrates content and chemical constituents (nitrogen, phosphorus and potassium percentages and contents/ plant) could be achieved by irrigating plants with the highest irrigation water rate (12 L/ plant/ week) combined  with spraying plants with CaCO₃ at 6%.