The present investigation was conducted to investigate the effects of biological and organic fertilization including cerealine, mycorrhizae and compost on growth, yield and oil percentage of canola plants grown under two levels of NPK mineral fertilizers, i.e. 50 and 100% of the recommended NPK. The experiments were carried out in the plastic green house of the Plant Physiology Division, Fac. of Agric., CairoUniv., Giza, Egypt, during two successive growing seasons; 2004-2005 and 2005-2006. The obtained results confirmed the positive effects of bio and organic fertilization on growth and yield of canola plants either under half or full NPK dose which recorded significant increments in comparison with only mineral fertilized plants. In addition, the treated plants with the combination of half NPK+ compost+ cerealine+ mycorrhizae were able to approach their optimal productivity under only full NPK. Moreover, the percentage of oil was increased in seeds of canola plants treated with the half NPK + the combinations of compost, compost+ cerealine, compost+ mycorrhizae and compost+ cerealine+ mycorrhizae in comparison with the control plants of full NPK treatment. Furthermore, the value of seed oil content (g\plant) of half NPK+ compost+ cerealine+ mycorrhizae was exceeded the comparable value of the control plants of full NPK. Therefore, these results strongly suggest that it could be reduce the amount of NPK mineral fertilizers to the half dose to achieve the same yield of canola plants treated with full NPK dose when compost and bio fertilizers were added, leading to economical and environmental benefits through reducing the cost and the hazardous effects of mineral fertilizers on the environment.