The experimental trail was consummated in Plant Tissue Culture Laboratory at El-Zohria Botanical Garden, Horticulture Research Institute, AgricultureResearchCenter, during 2007 and 2008 years. It was intended to find out the most suitable treatments for propagation of Gerbera jamesonii Boluscv Aurantiaca  by using the tissue culture technique. So, the study was done using explants from juvenile leaves and seeds of the plant. The results emphasized that using 30% Clorox for 15 min and 30% Clorox for 10-20 min, respectively for sterilization of explants gave the best result of (100%) survival and free of contamination of leaf and seeds explants. BA at 0.5 mg/l and 1.0 or 2.0 mg/l NAA were the best concentrations for callus formation from leaves. Furthermore, the use of BA on plantlet formation of seeds was not significant when BA was used at either 0.0 or 0.5 mg/l with the different concentrations of NAA and 1.0 mg/l BA plus either 2.0 or 4.0 mg/l NAA. Leaves explant was better than seeds for number of shoots when cultured on the multiplication medium. MS medium plus 1.0 mg/l Kin and 0.5 mg/l NAA was mor the most appropriate for shoot formation from leafs callus. MS medium plus 2.0 mg/l Kin was favoured for number of shoot from seeds. The shoots of Gerbera jamesonii Boluscv Aurantiaca were successfully rooted when cultured in MS medium supplemented with 1.5 mg/l IBA. Plantlets after root development exhibited 100% survival in plastic pots filled with peat moss and sand at a ratio of 1:1, 2:1 and 3:1 (by volume).