Response of Egyptian henbane plant callus (Hyoscyamus muticus L.) to various levels of salt was investigated. Two tolerant clones to salt were selected from callus cultured on selection medium contained 6000 and 8000 ppm of salts, respectively. Tolerant clones were capable of proliferating and growing on media contained the same salt concentrations and supplemented with 1.5 ppm of BA. Vegetative characters were significantly enhanced in general at the lower salt concentration (6000 ppm) especially with the media containing 0.12 g/ L KH₂PO₄ +4 ppm kinetin + 0.5 ppm IBA. Using of MS media at its half strength with 1ppm NAA was the best for henbane root growth at 6000ppm of salt. Survival percentage  reached  up to 28.00 % for clone A(selected  at 6000ppm ) and 32.02 % for clone  B(selected  at 8000ppm) with a total alkaloid percentage of  0.90  and 0.98 %, respectively .