Axillary buds Cultures of Digitalis lanata Ehrh were established from seedling, obtained under aseptic conditions. Shoot tip and single node explants were cultured on MS basal solid medium with various of BA concentrations. Shoots proliferation and growth were significantly influenced by BA levels and explant type. The highest amount of shoots was obtained on medium supplemented with 5.0 µM of BA, while digoxin and digitoxin contents affected by high level of BA (17.5 µM). A further subculture to medium containing high levels of BA promoted shoot growth and suppressed glycoside contents. Production of digoxin and digitoxin improved upon transferring shoots onto half strength on MS medium, vitrified shoots were appeared as well. Interestingly, addition progesterone as precursor, led to enhancement of digoxin and digitoxin contents, especially on rooted shoots.