L-asparaginase (EC 3.5.1.1) activity is measured in Cicer arietinum cotyledons. Studying the relation between germination time of Cicer and L-asparaginase activity throughout 7 days revealed that L-asparaginase activity increased gradually up to the 5^th day of germination after which it is declined.Jasmonic acid (JA) induced L-asparaginase activity and 100 µM was the best concentration for the induction.Treatment of cotyledons with 100 µM JA resulted in the increase of the activity increased throughout the experimental period.  The results showed that incubating the cotyledons with 100 µM GA₃ for 24 h resulted in the induction of the enzyme activity. However, incubation with 100 µM ABA for 24 h caused a reduction in the enzyme activity. The induction of L-asparaginase by GA₃ was time-dependent throughout the experimental period of 72 h. The results show that very low activity of the enzyme was recorded in cotyledons exposed to dark in absence of L-asparagine.Actinomycin D, cycloheximide and chloramphenicol inhibited the induction of L-asparaginase activity by GA₃ or kinetin in Cicer cotyledons.