The experiment was conducted at Agricultural Research Center Farm, Giza, Egypt during 2008 and 2009 seasons to estimate the genetic parameters for F₂ and F₃ generations in the two intra-specific crosses (G. 89 x G. 86) x Suvin and (G.89 x Pima S₆) x (G.75 x Sea Island). Observations were recorded on boll weight (g), seed cotton yield/plant (g), lint percentage (%), 2.5% span length (mm), fiber fineness, fiber strength and uniformity index traits. The analysis of variance for all traits studied manifested highly significant differences of F₃ generations in the two crosses, while, the F₂ generation exhibited highly significant for seed cotton yield/plant and uniformity index in the first cross, and boll weight, seed cotton yield/plant and uniformity index in the second cross. The traits, seed cotton yield, boll weight and lint percentage showed high phenotypic and genotypic coefficients of variations estimates for F₂ and F₃ generations in the two crosses. There is enough scope for selection based on these characters, and the diverse genotypes can provide materials for a sound breeding programme. High heritability coupled with high genetic advance and genetic advance as percentage of mean (genetic gain) observed for seed cotton yield/plant, boll weight and lint percentage of F₂ and F₃ generations in the two crosses showed that these traits were controlled by additive gene effects and phenotypic selection for these characters would likely to be effective. These results suggest that rigorous plant selection is required to identify desirable plants from F₂ and F₃ generation. Selection pressure is low in the F₂ and F₃ generations but increases in the F₄ generation for private breeders.