The present experiment included three growth and root promoting regulators, Indole butyric acid (IBA) solution at 4000 ppm, Hydrogen Peroxide (H₂O₂) solution at 3.5% and Vitamin B₁₂ solution at 24mg/L. They were used either solely or in combination along with their interactions with split wounding process at the basal portion of hardwood stem cuttings (5cm) detached from fruiting guava trees (Psidium guajava L.). Just before inserted the non-wounded and wounded cuttings in planting medium (Sand and Peatmoss 1:1 v/v), they were dipped in each of the tested solutions for 30 second. The control was untreated cuttings just pre-planting dipped in distilled water for 30 second. The effect of treatments under study on certain chemicals content in basal portion of the treated cuttings was tested. Such chemicals included level of nitrogen and carbohydrate contents, C/N ratio and phenol contents. The relationship among these chemical constituents in cuttings basal and their rooting ability was the main target of the current research. Rooting ability was estimated through the measurement of 4 root parameters, rooting percentage, number of adventitious primary roots, adventitious primary root length and number of secondary (lateral) roots per cutting. Results obtained in this experiment indicated that the chemical constituents in both non-wounded and wounded cuttings were significantly affected by various growth and root promoters along with the interactions between them. As for the estimated relationships, it was noticed that cuttings of T₅ [Dipping in (H₂O₂+IBA) and T₁₂ (Wounded followed by Dipping in H₂O₂ and in IBA)], which were determined an appropriate level of N contents, the high value of carbohydrates and C/N ratio as well as low phenols content, were the most favorable cuttings to record higher rooting percentage and to form adventitious roots of good qualities compared with those of the other treatments. On the other hand, both non-wounded and wounded cuttings which had non-appropriate level of N contents, the lower values of carbohydrates and C/N ratio along with higher phenols either completely failed to induce adventitious roots or tabulated significantly lower rooting percentage and the induced roots were of poor characteristics.