The cool germination test as a stress test is usually conducted to evaluate and rank the different cotton seed lots which are offered for sale to assess their planting value or to determine their storage potential for carry over. The cool germination test at 18ºC for 7 days can be used with confidence for Gossypium hirsutum L. but the applicability of this test to G. barbadense L. is not known. This research was conducted to investigate the effect of using different temperatures i.e. 16, 18 and 20ºC for 7 days on different seed samples from 2 seed classes i.e. breeder seed and foundation (basic) seed classes from 8 Egyptian cotton G. Barbadence L. varieties representing all the cultivated cotton varieties in Egypt during 2002 planting season.  In addition to determining the temperature which could be applied in the cool germination testing for the Egyptian cotton variety seeds, it is proposed that the emerged seedlings could be classified to 3 categories. The first category seedlings which already have achieved 4 cm or more as a combined root – hypocotyl length, measurement is made from the tip of the radicle (primary root) to the point of attachment to the cotyledons, these are considered as  high vigor or  strong seedlings.  The second category, or the medium vigor seedlings have 2-<4 cm combined length.  The last category or the low vigor seedlings have less than 2 cm as combined length. The findings of this study propose that using 20ºC for 7 days for G. barbadense L. can be applied with confidence in cool germination test as the percentage of high vigor seedlings or the total of the 3 categories were close or similar to the percentage germination.