The present investigation was conducted to study the effect of 3 solutions of growth and root-induced regulators, Indolebutyric acid (IBA) at 4000ppm, Hydrogen Peroxide (H2O2) at 3.5% and Vitamin B12 at 24mg/L along with wounding process, either solely or in interactions, on rooting ability of hardwood cuttings of guava cv. Balady (Psidium guajava L.). The basal portion of wounded and non-wounded cuttings just before inserted in the planting medium (Sand and Peatmoss 1:1 v/v) were dipped in these solutions for 30 second each.
Growth and root-induced regulators were significantly affected buds burst and survival percentages per cutting. A similar effect was shown on number of shoots and leaves per cutting at 1.5-month-old, along with rooting percentage, primary roots number, primary root length and secondary roots number per cutting at 3 month-old. Maximum average of buds burst % at 8-week-old (81.52%) and buds survival % at 12-week-old (62.83%) along with more number of shoots/cutting (3.43 shoots), more number of leaves/cutting (5.75 leaves), maximum rooting percentage (70.67%), more primary roots number/cutting (24.96 roots) and maximum primary root length/cutting (8.38cm) were noticed in hardwood wounded cuttings pre-planting treated with dipping in H2O2 solution at 3.5% and in IBA solution at 4000ppm for 30 second each (T12). More secondary roots number/cutting (11.34 roots) was tabulated in wounded cuttings treated with wounding process only (T8). The corresponding average results in non-wounded treated cuttings indicated that maximum buds burst percentage (72.65%) was in cuttings treated with H2O2 solution (T3). Maximum buds survival percentage (53.05%), more shoots number per cutting (2.55 shoots), more leaves number/cutting (4.89 leaves), maximum rooting percentage (43.00%) and more primary roots number/cutting (16.65 roots) were observed in cuttings treated with H2O2 at 3.5% and IBA at 4000ppm for 30 second each (T5). Maximum primary root length/cutting (7.10cm) and more secondary roots number/cutting (5.67 roots) were recorded for non-wounded cuttings treated with IBA at 4000ppm for 30 second (T2).