This study was conducted in orchard and greenhouse of the developing agricultural systems project, Faculty of Agriculture, Cairo University, in the period from 2006 to 2008 in order to improve the ability of rooting in Kalamata olive cv. twenty two years old Kalamata olive cv. trees were used as mother plants. One year old branches were used to prepare the cuttings in four dates (Middle of November, first of December, Middle of December, and first of January). Mechanical treatments (wounding) alone and with some growth regulator treatments were used to stimulate rooting ability. The study revealed that the best rooting for Kalamata cuttings were obtained when cuttings were prepared in the middle of November with two leaves and treated by wounding and beating the basal portion after dipping bases of cuttings in indole butyric acid solution (IBA) at 4500 ppm. Microscopical of the transverse sections showed that there were no clear differences between dates of preparing cuttings in sclerenchyma cells volume and count.