Two field experiments were conducted at the Experimental Station, Faculty of Agriculture, Mansoura University, during the two successive winter seasons of 2009/2010 and 2010/2011. The main objectives of this study were  determining the effect of sowing dates (1^st Sept., 1^st Oct. and 1^st Nov.), nitrogen fertilizer levels (40, 60 and 80 kg N/fed) and time of foliar application with "Melagrow" as natural growth promoter (without, one and two times) on growth and petal yield of safflower (Carthamus tinctorius, L.) cv. Giza 1. The obtained results could be summarized as follows: Intermediate sowing date of safflower (1^st October) significantly surpassed other sowing dates (1^st September and 1^st November) in all studied growth and petal yield characters, except Crop growth rate "CGR", Relative growth rate "RGR" and Net assimilation rate "NAR" which produced from sowing on 1^st September in both seasons.  Fertilizing safflower plants with 80 kg N/fed significantly increased all studied growth and petal yield and markedly recorded the highest values of these characters in both seasons. However, application of 40 kg N/fed produced the lowest values of all studied characters in the two growing seasons. Foliar application with Melagrow( as a natural growth promoter) twice after 30 and 70 days from sowing significantly recorded the highest values of studied growth and petal yield characters, exclusion leaves/stem and branches ratio, CRG, RGR and NAR in both seasons. While, application of Melagrow one time after 30 days from sowing came in the second rank in this respect in both seasons. On the other hand, the lowest values of all growth and petal yield characters were resulted from the control treatment (without foliar application) in both seasons. From the obtained data in this study, it can be concluded that sowing safflower on 1^st October and mineral fertilizing with 80 kg N/fed beside twice foliar application with Melagrow as a natural growth promoter in order to maximizing its growth and petal yield under the environmental conditions of Dakahlia Governorate, Egypt.