In vitro mass propagation and characterization (morphological and molecular, RAPD) of banana (Musa spp.) cultivar ‘Grand Naine' was carried out. Produced 40000 vitroplants were screened for somaclonal variations during acclimatization, nursery and open field stages. The total detected variants were grouped into 25 off-types (two of them were died) in addition to the normal plant. Random Amplified Polymorphic DNA (RAPD) was carried out to study the differences among the normal cultivar ‘Grand Naine' and its 23 variants using 17 arbitrary primers. Cluster analysis results revealed that ‘winged petiole' and ‘deformed lamina' were more related to the normal plant. However, ‘Giant plant' and ‘weak plant' related to each other and clustered with normal plant. According to principal coordinate analysis, most of the variants were aggregated nearly whereas ‘variegated plant' was separated apart from the other variants. This may reflect the genetic difference between ‘variegated plant' and the other variants. The obtained results from both molecular and morphological analyses were so far in contiguous with better resolution when using the PCOORDA analysis than cluster analysis.