This study was carried out in greenhouse at Sinai development project, Ministry of Agriculture, North Sinai, Egypt, for three months to investigate the effect of the irrigation by different treatments (0,25,50,75 and100%) of bio-treated tannery wastewater on growth parameters of three shrubs and seven timber trees seedlings. The results showed that a gradual reduction in growth and biomass production of different seedlings with increasing application of Cr (VI) in irrigation water. Hence, the total dry weight was high significant different between control and different treatment of  Cr(VI), but the irrigation by 25% bio-treated tannery wastewater gave the high growth parameter and the total dry weight reached 2.054 gm to followed by the other treatments.         There were a gradual increase in Cr (VI) concentration in shoot, root, total uptake and translocation from root to shoot with increasing application of Cr (VI) in irrigation water. The irrigation with 100 % bio-treated tannery wastewater gave the high chromium concentration in shoot, root, total and total uptake (14.82, 37.21, 52.01 and 71.16 µg g^-1, respectively) followed by other treatments. However, there was no significance difference between translocation from root to shoot of chromium with different treatments, and there is no effect on the percentage of infection by VAM under high concentration of Cr (VI). Different seedling show different  tolerance levels of Cr (VI) pollution. Pinus halepensis is the most sensitive species to Cr (VI). In contrast Jatropha curcas, Pongamia pinnata and Albizzia lebbek are the most resist and hyperaccumulation species, and chromium accumulation in seedlings tissues was in order of roots › shoots. So, it was suggesting that these species could be employed in phytoremedion of soils contaminated with Cr (VI), and recycling of bio-treated tannery wastewater in shrubs and timber trees nursery.