This study was carried out to distinguish among eight soybean  (Glycine max L.) cultivars using biochemical and molecular marker. Therefore, SDS-storage proteins, some isozyme (esterase (EST), peroxidase (Prx) and glutamate oxaloacetate transaminase (GOT)) and PCR-RAPD (polymerase chain reaction-randomly amplified polymorphic DNA) markers were carried out. The results of protein electrophoresis revealed that total number of bands ranged from 10 in cultivar G.21 to 14 in cultivar Crawford.. Nine common bands were found in all cultivars. Some cultivars showed specific bands which could be used to distinguish between others. Each of G.22, G.35, G.21, G.111 and G.83cultivars had only unique band, with molecular weight of 53.83, 44.84, 33.37, 21.66 and 16.73 kda, respectively. Bands of three isozymes systems (Est,Prx and GOT) were determined for soybean cultivars identification based on polyacrylamide gel electrophoresis. Using the number of bands, Rf values and its densities can be used to identify these varieties. One polymorphic Est isozyme was found band in cultivar Giza 21 with RF about 0.579 this band considered as a marker for this cultivar. A total number of two Prx isozyme bands ware detected in two band was monomorphic RF 0.441 and 0.569 with different band densities in height densities in cultivars Giza35 and Giza 83. A total number of two GOT isozyme bands ware monomorphic RF 0.556 and 0.721 with different densities and intensities.        While molecular marker RAPD analysis utilizes six random primer the largest number of RAPD specific markers was generated by primer O02 (four markers) followed by O08 (three markers). While primers B04 and B12 generated two markers the lowest number of RAPD specific markers was generated by primers A12 and B06 (one marker). The largest number of RAPD specific markers were scored for cultivar Giza 21 (7 markers) followed by Giza 35 (3 markers).