Six male genetic-cytoplasmic sterile lines (ICSA-14, ICSA-44, ICSA-93, ICSA-155, ICSA-88010and ICSA-ATX2-2) and six testers (ICSR-93002, ICSR-93004, ICSR-91022, ICSR-89025, ICSR-89028 and ICSR-93018) were crossed in line x tester design in 2008 season at Shandweel Agric. Res. Station, Sohag, Egypt to produce 36 hybrid combinations. These hybrids along with their twelve parents were grown in a randomized block design with three replications in 2009 and 2010 seasons. The samples for estimation of four characters (50% flowering, plant height (cm), 1000-grain weight (gm) and grain yield/plant.) were taken. The analysis of variance indicated significant differences for the studied characters in all the genotypes, comparing with local and check hybrid. The obtained data also clearly show that the crosses had highly significant differences among all studies traits at two growing seasons 2009 & 2010 and combined over the two seasons, the cross ICSA-ATX2-2× ICSR93002 was earliest genotype and it has lowest negative heterosis relative to better parent, the combination ICSA-14 × ICSR93004 was the best cross in plant height trait and the cross ICSA-93× ICSR-93018 recorded highest value of heteosis of 1000-grain weight, while the cross ICSA-ATX2-2 × ICSR93004 was the best cross for grain yield/plant. The parents ICSR-93002, ICSA-93, ICSA-88010 and ICSA-ATX2-2 were good combiners for 50% flowering and the parents ICSR-93002, ICSR-89028, ICSR-93018, ICSA-14 and ICSA-44 were good combiners for plant height and the parents  ICSR-93002, ICSR-93004,  ICSR-93018 and ICSA-93 were good combiners for 1000-grain weight while the parents ICSR-93004, ICSR-93018, ICSA-155, ICSA-88010 and ICSA-ATX2-2 were good combiners for grain yield/plant. Some hybrids had positive sca effects for some traits, but ICSA-88010×ICSR-91022 was the only hybrid which had negative highly significant specific combining ability for 50% flowering. For plant height, the crosses ICSA-93×ICSR93002, ICSA-ATX2 -2 × ICSR-89028 and ICSA-88010 × ICSR-91022 had highly positive sca effects, respectively. The crosses ICSA-44×ICSR-89025, ICSA-14×ICSR-89028 and ICSA-ATX2-2×ICSR93004 were observed and had highly positive sca effects for1000-grain weight. For grain yield/plant, The crosses ICSA-88010 × ICSR-89028, ICSA-14 × ICSR-89028 and ICSA-44 × ICSR93002 showed the greatest positive sca effect. These combinations could be utilized in the hybrids programs of grain
 sorghum