The present investigation was carried out at Shandaweel Agricultural Research Station, Sohag Governorate to study the performance of the two promising sugar cane varieties grown as plant cane in 2008/2009 and 2009/2010 seasons and the 1^st ratoon crops in 2009/2010 under three row spacing of (80, 100 (the recommended) and 120 cm) and two promising sugar cane varieties, G.98-28, G.99-160 beside G.T.54-9 the commercial variety. A split plot design with four replications was used in the plant cane and 1^st ratoon crops. Row spacing treatments were allocated in the main plots, while, sugar cane varieties were randomely distributed in the sub-plots. The results showed that: Increasing row spacing up to 120 cm recorded the highest values of stalk height, diameter, brix%, sucrose%, purity%, no. of millable canes/fed, sugar recovery%, cane and sugar yields/fed in the plant cane and 1^st ratoon crops compared with the other two spacing. Sugarcane varieties differed significantly, where G.98-28 variety recorded the highest stalk height, sucrose%, purity%, no. of millable canes/fed, sugar recovery%, cane and sugar yields/fed in the plant cane and 1^st ratoon crops over the other two varieties. G.T.54-9 variety surpassed G.98-28 and G.99-160 varieties in stalk diameter, brix% in the plant cane and 1^st ratoon crops. The interaction between G.98-28 variety and 120 cm interrow spacing in 1^st season plant cane and 1^st ratoon crops obtained the highest cane and sugar yields/fed. Under this conditions, 120 cm row spacing for the plant cane and the 1^st ratoon crop of G.98-28 variety grown at Shandaweel, can be recommended to obtain the higher cane and sugar yields/fed.