Two field experiments were carried out at Gemmeiza Agricultural Research Station, Gharbia Governorate during two seasons (2008 and 2009) to study the effect of four aminototal concentrations (i.e without, 1g/L, 2g/L and 3g/L) under three N rates (15, 30. and 45 Kg N/fad.) and their interaction on leaf chemical composition, seed and fiber quality of the Egyptian cotton cultivar, Giza 86. Results indicated that the differences between N rates for leaf N content, seed index, protein  and oil % were significant, where, lint %, micronaire reading and pressley index were insignificant in the two seasons. Rate of N fertilizer 45 kg N/fad. gave the highest values for leaf N content, seed index, protein  and oil %, while, 30 kg N/fad. gave the highest values for leaf k content in the both seasons. Foliar application of aminototal gave a significant effect on leaf N and K contents, seed index, protein  and oil %, on the other hand, lint %, micronaire reading and Pressley index were insignificant in the two seasons. Foliar cotton plants with 3g/L or 2g/L concentration from aminototal gave the highest values of most studied attributes. The interaction between N rates and aminototal concentrations were significant for most studied traits except lint %, micronair reading and Pressley index in the two seasons. Also, results show that, fertilization cotton plants with 30 kg N/fad. and sprayed with 2 g/L aminototal improved the fiber and seed quality under the experiment soil conditions.