The main objective of this study is: Effect of two treatment of bacterial endophytes strains Azotobacter chrocooccum (E1) and Pseudomonas sp. (E2) individually whether as grains soaking and foliar application on some physiological parameters of two wheat plants (Triticum aestivum L.) cultivars (Sakha 93 and Gmiza 9) grown under three levels of irrigation water deficit stress 75, 50 and 25 % field capacity. The tested physiological parameters were chlorophyll pigment (chl. a, b and total) contents, relative water content (RWC), leaf water content (LWC), leaf water deficit (LWD), proline content and some major  essential elements (NPK) contents. Negative impacts were obtained on the tested wheat cultivars grown under the different irrigation water deficit. Application of Azotobacter chrocooccum (E1) and Pseudomonas sp. (E2) strains individually were carried out by spray foliar and grains soaking treatments increased the tested physiological parameters for two cultivars compared with untreated plant, which could overcome the negative effects of drought stress.