Two filed experiments were conducted during the growing seasons 2008/2009 and 2009/2010 at Agricultural Research Station in Omar Al-Mukhtar University to investigate the response yield and its components of two six rows barley varieties (Wadi El-Koof and Giza 123) and one two rows Varity (Giza 128) three sowing dates (mid Nov., mid Dec. and mid Jan.) at El-Baida, Libya condition. The obtained results indicated that the effect of sowing dates was significant on spike weight and biological yield in both seasons, and it was significant on main stem height in the first season, as well as was significant on tillers number/m², grain yield/ha and harvest index in second season, but it was insignificant on spikes number /m², grain number /spike, straw yield/ha and seed index in both seasons. Sowing date of mid December gave the highest grain yield/ ha compared to other sowing dates. Result showed that barley varieties significant differed in spike number/m2 and spike weight in 2009/2010 season only and significant differed in all other traits in both seasons. The interaction effect between sowing dates and barley varieties was insignificant on all studied characters in both seasons.