Two field experiments were conducted during 2008/2009 and 2009/2010 seasons under new irrigation system (Sprinkler irrigation) at the Experimental Farm of Ismailia Agric Res. Station, ARC, to investigate the impact of fertilization treatments (in terms of mineral N levels either alone or combined with Nitrobein (bio- N fertilizer) on yield quantity and its quality as well as analysis of some flax genotypes i.e. Sakha 3, Viking, Sakha 2 and the flax strain 2419/1. The main finds could be summarized as follows:   The results showed that flax Sakha 3 variety ranked first and surpassed the other three genotypes in straw yield characters. While, flax 2419/1 strain achieved highest estimates regarding seed yield characters. There was gradual increments as increasing nitrogen levels either in alone case or combined with Nitrobein (bio-fertilizer) concerning all straw and seed yield characters except for fiber fineness which was decreased. The difference between nitrogen treatment at 60 kg mineral nitrogen /fad + Nitrobein and added 75 kg N/fad did not reach the level of significant in most characters especially fiber, seed and oil yields/fad. The interaction between genotypes and nitrogenous fertilization treatments had significance effect on straw and fiber yield/fed, fiber fineness, 1000 seed weight as well as seed and oil yield/fad. Results revealed positive and significant correlation values between straw yield/fad and each of technical length/ plant, straw yield/plant, fiber yield/fad as well as per plant, fiber length and long fiber%, also between seed yield/fad and each of seed yield/plant, No. of capsules/plant, No. of seeds/capsule, 1000 seed weight and seed oil%. Path coefficient analysis indicated that the long fiber percentage, fiber yield/plant and their interaction considered as the most important characters to increase fiber yield/fad. In the same time, seed yield/plant, No. of seeds/capsule and their interaction had similar effect for increasing seed yield/fad within the studied four flax genotypes. It could be summarized that for maximizing the productivity with best quality, minimizing the production cost of mineral fertilizer and environmental pollution for the tested flax genotypes, it could be recommended to inoculating Sakha 3 variety seed with 1.5kg/fad Nitrobein and fertilized their plants with 60kg/fad mineral nitrogen.