The aim of this study was to investigate the response of two faba bean cultivars (Misr-1 and Giza-40) to bio-fertilizers (Bacillus megatherium  var. phosphaticum and Psedomonas) and foliar spraying with some micro- elements (Fe- Zn and Mn) on growth, yield and seeds quality. Two field experiments were carried out during winter seasons of 2017/2018 and 2018/2019 at the Experimental Farm of Agricultural Res. Center, Al-Azhar University , Assuit  Governorate, Egypt. Split-split plot design was used with three replicates , where faba bean cultivars were assigned to the main plots , foliar spraying were distributed randomaly in the sub plots and bio-fertilizers were located in the sub-sub plots . Results concluded that faba bean cultivars exhibited significant difference in plant height, number of branches/plant, number of pods/plant, seed yield/plant, 100-seed weight , straw yield/fed , protein and phosphorus percentage of seeds in both seasons while faba bean cultivars exhibited significant difference in seed yield/fed in first season only. Where, cultivar Misr-1 superior to Giza-40 in all studied characters. Also, micro elements foliar application caused a signification increases in all studied characters. Where, the highest values were recorded with Fe spraying in both seasons. Also, application of phosphorein induced significant increases in all previous  characters in both seasons. Therefore , this study recommends that Misr-1 cultivar inoculation with phosphorein and foliar spraying with Iron  in order to improve the production  of faba bean under the condition of Assuit Governorate, Egypt.