A laboratory experiment was carried out at Seed Testing Laboratory, Agronomy Department, Faculty of Agriculture, Mansoura University, Egypt, during November and December 2018 to study the effects of application methods and seeds treatments with some biostimulants on germination parameters of bread wheat Misr 2 cultivar. The experiment was conducted in factorial experiment in Completely Randomized Design (CRD) with 4 replications. The experiment included two factors. The first factor included two application methods with some biostimulants (soaking and priming). The second factor integrated with eleven seed treatments with biostimulant substances. The obtained results showed that the priming method surpassed soaking method in final germination percentage, germination index and co-efficient of germination. Nevertheless, soaking method surpassed priming method in abnormal seedlings %, speed germination index, mean germination time and germination energy %. Treating seeds with yeast extract surpassed the other studied treatments in speed germination index, mean germination time and germination energy percentage. Conversely, treating with mixture of ascorbic acid and salicylic acid  surpassed other studied treatments in final germination percentage and germination index. However, treating with mixture of humic acid + amino acid + algea extract + yeast extract surpassed other studied treatments in abnormal seedlings percentage. It could be recommended that priming seeds of bread wheat Misr 2 cultivar with the mixture of ascorbic acid and salicylic acid at the rate of 200 mg/L of each or yeast extract at the rate of 100/L as an effective way for improving germination parameters.