The objectives of this study were to determine the heterosis, combining abilities, proportional contributions, genetic components and heritability measurements of some yield, its components and fiber quality characters for six genotypes belong to Gossypium barbadense, L viz., Giza 95 (L₁), Giza 87 (L₂), and Giza 93 (L₃), which used as a parental lines (L) as well as, Suvin (T₁), Karshenky (T₂), and 10229 (T₃) which used as a testers (T), by using line x tester analysis (LxT) across three locations i.e. Sakha, Sids and Shandweel during 2017 and 2018 seasons. The variances due to the genotypes, locations (Loc.), parents (P), crosses (C), (P vs. C), L, T, (parents x loc.) and (P vs. C x loc.) were significant for most traits under study. Among the parents, Giza 95 (L₁) and Karshenky (T₂) were highest yielding parents for most studied yield traits, Giza 87 (L₂) and Suvin (T₁) for most studied fiber traits. According to useful heterosis and desirable specific combining ability effects estimations, the promising recombination's i.e., (L₁xT₁), (L₂xT₂), (L₃xT₁), (L₃xT₂) and (L₃xT₃) were the highest values for most studied traits. Proportion contribution of testers were the high values for No. of bolls/plant, seed cotton yield per plant, boll weight and fiber strength traits. Meanwhile, lines contributions were the highest for other traits. The non-additive genetic effect was larger than additive ones for NB/P, SCY/P, LY/P and length uniformity index. Meanwhile, heritability in broad sense (h²_b %) were higher than the values of narrow sense (h²_n %) for all characters under study.