The present investigation was carried out at two summer seasons of 2011 and 2012 at two different locations. Sids Research Station in Upper Egypt and Sakha Research Station in North Delta of the Agricultural Research Center, Egypt. Each experiment was designed as a randomized complete block design with three replications. The objective of this investigation is aimed to study the behavior of genetic interspecific of maize-teosinte crosses under two locations.                 Twenty one crosses were made among three teosinte (Euchlaena maxicana) as male tester parents and seven genotypes (Zea mays) as female maize parents in 2011 season in 2012 summer season. The 21 F₁ and their ten parents were planted in two experiments in the two locations (Sakha and Sids stations). Main finding could be summarized as follows: 1-Genotypes appeared to be varied from location to another with respect to their means for most of the studied characters. 2-The parent Maize SC10 had highest and desirable means for all the studied characters and tester Sakha (teosinte) produced highest means for all studied traits. 3-The crosses (SC168 x inbred lines) (SC125 x Sakha) and (inbred line 7 x inbred line 3) were the best for all the studied traits. 4-The best desirable GCA effects in maize fresh yield in kg/plant, stem diameter and dry matter % were found in the single cross 168. The favorable fresh yield in kg/plant was inbred line 34 for number of leaves/plant were inbred line 7 and the two parent inbred line 7 and SC124 for number of leaves/plant. 5-The results indicated that the desirable general combining ability effects teosinte for dry mater % and dry yield/plant in Damietta parent and the desirable for number of leaves/plant for parent line 3 and Sakha. 6-The results revealed that the best desirable estimates of SCA for fresh yield in kg/plant, number of stems/plant, dry matter and number of leaves/plant were the cross (L₂ x T₁) .                 In general, the best desirable for most of the studied characters were parents SC168 and  inbred line 3 for breeding programs