A field experiment was carried out during the two successive seasons of 2011/2012 and 2012/2013 to study the effect of compost levels (zero, 5, 10, 15 ton/fed.) and biofertilizers (effective microorganisms and active yeast), as well as, some vitamins (vitamin E and vitamin B₁) and their interaction on Gladiolus grandiflorus cv. Eurovision plant. Results showed that vegetative growth (leaf length, number of leaves/plant and dry weight of leaves/plant), flowering aspects (length of spike, number of florets/spike and lower floret diameter) and corm production (corm diameter, corm dry weight and number of cormels per plant) were gradually increased by increasing the level of compost fertilizer.All biofertilizers and vitamins treatments significantly increased all vegetative growth characters, flowering parameters and corm and cormels production in comparison with the control. Effective microorganisms and active yeast treatment seemed to be more effective than other treatments in this concern. Their use of high level of compost (15 ton/fed.) in combination with combined biofertilizers treatment noticeably improved different vegetative growth characters, flowering parameters and corm production of gladiolus.