Beta
74484

IN VITRO PROPAGATION METHODES OF SNAPDRAGON (Antirrhinum majus.L) PLANT.

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

-

Abstract

The present investigation was carried out to study the direct and indirectmicropropagation of Antirrhinum majus In order to reduce the cost of production and to ensure the production of genetically identical ornamental plantlets for further field culture as well as for medium- term conservation for future use and research. For the direct micropropagation addition of 70.0 % Ethyl alcohol for 30 sec and 30.0% commercial Clorox (1.30% NaOCl) for 5.00 minute were the best treatment for seeds sterilization. Hypocotyl, cotyledon and cotyledonary node were excised from in vitro grown seedlings.  These explants were cultured for adventitious shoot regeneration. Using MS medium supplemented with BAP at 0.50 and 1.00 mg/L produced the highest shoots number of 13.50 shoots for both of them. Microshoots were rooted on MS medium containing IBA, IAA or NAA at 0.0, 0.50, 1.00 or 2.00 mg/L. Ninety percent of the microshoots were rooted on MS medium supplemented with 0.50 and 1.0 mg/L IBA. The rooting which achieved on medium fortified with NAA at 0.50 mg/L significantly recorded the highest roots number of 12.83 roots. A total of 90% survival was achieved and an increase in shoots length (12.71cm) when rooted explants were acclimatized ex vitro using 1: 1 soil: vermiculite mixture. On the other hand, for indirect micropropagation, explants successfully formed callus by using MS medium supplemented with TDZ at 1.00, 2.00 or 4.00 mg/L. The highest shoots number derived from callus was recorded for callus cultured on MS medium fortified with 2.00 mg/L TDZ + 0.50 mg/L NAA.

DOI

10.21608/jpp.2013.74484

Keywords

2,4-D, 2,4-dichlorophenoxyacetic acid, BAP, 6-benzylamino purine, IBA, 3-indolebutyric acid, MS, Murashige and Skoog (1962) basal medium, NAA, Naphthaleneacetic acid, TDZ, Thidiazuron, Kin, N6-furfuryladenine (Kinetin), IAA, indole acetic acid

Authors

First Name

A.

Last Name

Hamza

MiddleName

M.

Affiliation

Veget and Flori Department., Fac. Of Agric., Mansoura Univ.

Email

-

City

-

Orcid

-

First Name

O.

Last Name

Abd El-Kafie

MiddleName

M.

Affiliation

Veget and Flori Department., Fac. Of Agric., Mansoura Univ.

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Helaly

MiddleName

A.

Affiliation

Veget and Flori Department., Fac. Of Agric., Mansoura Univ.

Email

-

City

-

Orcid

-

First Name

M.

Last Name

EL-Mongy

MiddleName

S.

Affiliation

Veget and Flori Department., Fac. Of Agric., Mansoura Univ.

Email

-

City

-

Orcid

-

Volume

4

Article Issue

11

Related Issue

11217

Issue Date

2013-11-01

Receive Date

2020-02-29

Publish Date

2013-11-01

Page Start

1,621

Page End

1,637

Print ISSN

2090-3669

Online ISSN

2090-374X

Link

https://jpp.journals.ekb.eg/article_74484.html

Detail API

https://jpp.journals.ekb.eg/service?article_code=74484

Order

4

Type

Original Article

Type Code

887

Publication Type

Journal

Publication Title

Journal of Plant Production

Publication Link

https://jpp.journals.ekb.eg/

MainTitle

IN VITRO PROPAGATION METHODES OF SNAPDRAGON (Antirrhinum majus.L) PLANT.

Details

Type

Article

Created At

22 Jan 2023