Two field experiments were conducted at the exp. Res. Stat. Fac. of Agric., Cairo Univ, Giza, Egypt during 2011and 2012seasons to investigate the effect of seed inculcation with Rizobactrein and/or organic fertilizer(Compost) computed with control treatment under two level of nitrogen fertilizer on growth, yield productivity, Seed quality  ,fiber  properties and same chemical constituents  of cotton. Results showed, that fertilizer treatment under study had a significant effect on all studied traits where as the best treatment was use of Rhizobactrein + Compost and75% N which give the best result in plant height, No. of sympodial branches, No. of open bolls/plant, boll weight, seed index, seed cotton yield per plant and per fedden, lint percentage and Earliness percentage. Oil and protein percentage significantly affected by fertilizer treatment whereas Rhizobactrein + Compost and75% N application give the highest values of oil and protein percentage. Fiber length,fiber strength and micronaire reading didn't significantly differ as affected by fertilizer treatment in both seasons.  Use of Rhizobactrein + Compost and 75% N increased chlorophyll a & b and total, total soluble suger, carotenoid and Net income/ fed. From the present study it could be concluded that, use of biofertilizer, Rhizabactrein (200g/30kg seed/fed), and compost with 75% N produce high growth and yield components.