Nutritional field trials were applied at the agriculture farm of the Third Field Army, Suez Governorate, Egypt country, during the seasons of 2016 to 2017 and 2017 to 2018 aiming to investigate the different sources of organic fertilization (farmyard, goats and compost) also some bio-fertilization (active dry yeast, microbein, Potassumag, NPK natural, and biohyomean) effect as well as the effect of their combinations on vegetative product, herb yield, essential oil production also chemical composition of Thyme plants [Thymus vulgaris,L]. The obtained results exhibited that all fertilizer treatments in general improved the studied vegetative growth and yield characters as well as chemical composition of plants compared to the control treatment in two seasons. The results showed also that plant biomass responded linearly to organic fertilizers application and the highest values of measurements in both seasons were realized with compost application at rate of 20 m3/fed. followed by goats at rate of 20 m3/fed. and FYM at rate of 20 m3/fed as the lowest ones in comparison with those of control plants in both seasons. Also, it is evident that the application of organic fertilization from different sources at the rate of 20 m3/fed., in combinations with the studied bio-fertilizers significantly improved in most cases all vegetative growth characters and essential oil production as well as chemical composition of thyme plants compared with those of control treatment. The highest values in this regard were realized with combination of compost at the rate of 20 m3/fed. plus microbein, potassumag, yeast and biohyomean in addition to the recommended chemical NPK. This treatment can be recommended for thyme plants in order to obtain a higher herb yield and essential oil production with better specifications.