The field experiments carried out in Ibshway (clay loam soil L1) and Tamiya ( loam sandy soil L2) districts, Fayoum Governorate, Egypt, during 2016 and 2017 seasons to study the effect of water sources (Fish culture water, and Nile water)  and nitrogen fertilization levels((20 – 40 – 60) kg N/ fed) on productivity of cowpea plants in two soil culture. Fish culture water (W₁) treatment had higher values than other Nile water (W₂) treatment in all aforementioned traits.N₆₀ application have the  significant maximum effects by (62.6 & 61.5%), (20.3 & 15.7%), (1(0.6 & 15.0%), (25.4 & 28.7%), (19.6 & 15.3%), (26.1 & 26.0%) and (10.5 & 11.6%) over the corresponding low nitrogen rate (N₂₀) treatment for Dry matter (g), Plant height (cm), No of pods, Pod length, Average 100-seeds weight, Yield and Protein (%) in both 1^st and 2^nd seasons, respectively in clay loam location. The combined analysis for two locations i.e. clay loam and  loam sandy soil indicates significant differences between locations for 100-seeds weight (g), Yield/fed (kg), Dry matter and Protein (%). Also, highly significant differences among both water sources and nitrogen rates were existed for all studied traits except 100-seed weight which was not significantly affected by water sources. Cowpea plants cv. Teba fertilized with 60 kg N/fed. and irrigated by fish culture water produced significantly highest values for all studied traits in Ibshway (clay loam soil) compared with Tamiya (  loam sandy soil) location with significantly differences between the two land types except pod length and100-seeds weight which exhibited no significant differences. On contrast, no significant differences were observed between the two locations (both old and new land types) for plant height, pod length, number of pods and 100 seeds weight when cowpea plants cv. Tiba fertilized with 60 kg N/fed. and irrigated with Nile water. The cowpea seeds irrigated with fish farm water contained some heavy metals namely lead, aluminum, arsenic, lithium and selenium. The concentration of each of these elements varied depending on the growing season and soil nature.