North Carolina designs are very effective in breaking undesirable linkage and lead to creating genetic variability in a population. F₁ population was obtained by crossing the two cultivars Giza 88 P₁ and Australian P₂. Followed crossing between F₂ with the original parents, P₁ and P₂. The analysis of variance was highly significant for all studied traits. While, females sets were highly significant for all studied traits, which exhibit difference between them. Also, the males were significant for boll weight (BW) g /plant, seed cotton yield (SCY) and lint yield g/plant(LY) g/plant, which showed difference between them. The interactions between them were highly significant for boll weight (BW) and seed cotton yield (SCY). That showed variation background. The additive variance was higher than dominance for most studied traits. The contribution of male or female parents was more pronounced in the genetic variation. Additive components of variation were higher than dominance ones for most studied traits reflecting decreased (√D/A) values (less than unity). While the dominance portion of the genetic variation played a role for some traits reflecting higher (√D/A) values than unity for boll weight (BW) and fiber length (FL.2.5%).