The present study was conducted to assess the effect of polyploidy induction on different properties of Cercis siliquastrum plant. Experiment was conducted in RCBD with 15 treatments and 3 replications. The seeds were treated with 0.0, 0.5, 1.0, 1.5 and 2.0 % colchicine solution at room temperature for various exposure times 12, 24 and 48 h. Observations on morphological variations were recorded on each plant in each treatment. Various in seed germination, morphological and growth characteristics observed due to effects of interactions between colchicine concentration and soaked period. By increasing of the colchicine concentration the seed germination rate decreased significantly from 80% to 50%,  the effects on seed germinate were most evident at the higher colchicine concentrations (1.5 to 2.0 %). Longer stem and root, thicker stem and root diameter, higher number of leaves, branch and roots per transplant, were width area, thick and greener leaves, variable anatomy wood stem were achieved and development as compared to control plants. The length and width of stomata (21.6 and 10.3µm) respectively were significantly increased and induced in 0.1% of colchicine for 48h, whereas stomata density was decreased to 68 per area in 0.2% of colchicine at 48h. in contrast with initial diploid plants. However 1.5 % and 0.2% concentrations of colchicine at different soaked time were more effective for producing variation in plants. These polyploids plants may be helpful for further development and improvement of ornamental tree.