In vitro rapid propagation of pear rootstock (Pyrus betulaefolia) was established and achieved from shoot tips and nodal segment explants of mature tree grown in the greenhouse. Explants cultured on MS (Murashige and Skoog, 1962) basal medium in addition to  Ca-pantothenate (10 mg/L) supplementedwith two types of cytokinins (BA or Kin ) at the concentration of 0.0, 1.0, 2.0 and 3.0 mg/L singly or combined with IBA at 0.0, 0.5 and 1.0 mg/L were used. Shoot tip explant recorded the highest shoots number (7.6/shoot) as compared with single node explant (5.0/shoot) under the same BA concentrations (1.0 mg/L) and absence of IBA after 6 weeks of incubation at 25 ± 1ºC  and 16 h photoperiod with a light intensity of 1500 lux using florescent lamps. Addition of cytokinin to the culture media was considered as limiting factor in shoot proliferation and was effective with the two explant types at low concentration of BA (1.0 mg/L) as compared with high concentrations (2.0 and 3.0 mg/L). Raised either BA or IBA concentrations alone or in combinations in the media reversely recorded lower shoot numbers in the two explant types compared with low concentration. Interestingly, single node that cultured on the medium contained BA (3.0 mg/L) and IBA (1.0 mg/L) obtained the lowest shoot number (2.4/shoot) as compared with all other studied treatments. The results revealed that BA was more effective than Kin in shoot formation at the same concentrations especially at 1.0 mg/L, since the number of shoots/explant (5.08) for BA against (3.23) for Kin. In the rooting stage, in general IBA  was found more superior than NAA in root characters. Healthy shoots separated individually  from the shoot clump and cultured on MS basal medium supplemented with IBA (0.5 mg/L) recorded the highest root formation (100 %), number of roots (5.7) as well as root length (2.5 cm) after 4 weeks of incubation. Well–developed pear plantlets transferred from rooting medium to acclimatization and the growing mixture of peat moss and perlite (1:1, v/v) obtained the highest plant survival (91.7 %), number of leaves (13.1 leaf) and plant height (17.7 cm) after 2 months in acclimatization.