This study aimed to test the influence of different carbon sources in woody plant medium with various salt strengths on in vitro propagation of oak. The results showed that, sucrose was better than the other sugars by giving the highest shoots number, length of shoots and leaves number per explant (2.05, 1.30 and 17.67 respectively). The highest shoots number per explant was achieved when 45 gl^-l of glucose and 30 gl^-l of sucrose were added by giving 3.44 and 3.33 shoots/ explant respectively followed by 45 gl^-l sucrose and 30 gl^-l of glucose by giving 2.67 and 2.11 shoots/ explant respectively. On the other hand, full Woody Plant Medium salt strength gave the best multiplication results while the quarter Woody Plant Medium salts strength gave the lowest values. The highest roots number per explant was achieved when full salts strength was used which gave 5.40 roots followed by half salts strength with 2.60 roots then quarter salts strength by 0.30 root which was the lowest parameter. Furthermore, the highest rooting percentage was acquired in both full and half salts strength which was 100% and the lowest rooting rate was 30% in quarter salts strength. Sucrose was the best carbon source by giving the best root system and giving the highest rooting percentage (100%) followed by glucose (60%). The produced oak plantlets were successfully hardened and acclimatized to be transferred to the permanent field in the greenhouse with a success reached 85%.