In order to study the effect of genotype × environment interaction and stability of sugar beet genotypes for seven cultivars, viz Lilly, DS 9004, Gazella, Oscar Poly, Pather, Toro and Hercule. A field trail was sown in eight environments as major four locations (Sakha, Giza, El-Fayoum and Malawy) for two years (2011/12 and 2012/13) using a randomized complete block design, with three replications. Analysis of variance for root yield, sugar yield and sugar content showed that the environment and genotype and genotype × environment interaction (GEI) were significant. GEI were evaluated by two methods (phenotypic stability and AMMI model). According to phenotypic stability analysis results, genotype (Lilly) was the most stable for sugar content and root and sugar yield. This genotype recorded the highest root and sugar yield (30.34 and 5.22 ton/fed, respectively) across environments, and Sakha environment had the highest mean values of environments followed by El-Fayoum environment. AMMI model explained most of the genotype × environment interaction (85.97%, 83.34 % and 86.47 %) for root yield, sugar content and, sugar yield, respectively. Lilly was the best genotype based on the biplot, and showed specific adaptation to Sakha and El-Fayoum location. The varieties Pather, Hercule and Toro were the lowest variety among the evaluated varieties and it is better not to use it in the studied areas. The genotypes Gazella, Oscar poly and DS9004 had an average genetic potential for the studied traits, but its high general adaptability, then it could be introduced for all areas. Among the locations, Sakha was the best location, and was more similar to El-Fayoum. Meanwhile, Malawy was the poorest location. Therefore, two stability methods confirmed that Sakha and El-Fayoum are recommended as suitable regions for sowing sugar beet and Lilly variety could be suggested as the best genotype for these locations. Meanwhile, AMMI method showed new information.