Two different salt sensitive Barley cultivars ( Wide Otpa and Arrayhan) were examined under three different  NaCl concentrations, 0.0, 100, and 300 mM.  Barley seeds were pretreated with two Gibberillic acid (GA₃) 0.0, And 100 μM concentrations were used. The papertowel (paper roll) method was used to germinate the seeds. Root length, shoot length, root mass, shoot mass and percentage seed germination, and relative water content were measured as indicators of hormone effects at different salt levels.  The results of this study indicate that the use of GA₃ as Barley pretreatment could possibly alleviate the damaging effects of high salt levels on seedlings, particularly in brackish soil. Wide Otpa cultivar was shown more responses to the hormone treatments than the other cultivar, particularly those plants were germinated under 100 mM NaCl.