Two field experiments were conducted at El-Hamoul area, Kafr El-Sheikh Governorate, Egypt during 2010/2011 and 2011/2012 seasons, to study the effect of six biofertilization treatments (Without biofertilization (control),inoculation wheat seeds with Nitrobine (450 g/feddan) 100% of the recommended dose , Nitrobine (675 g/feddan) 150% from the recommended dose, Cerialine (450 g/feddan) recommended dose and Cerialine (675 g/feddan) 150% from recommended dose) and four nitrogen rates (0, 30, 50 and 70kg N/feddan.) and their interactions on growth, yield and quality of wheat . -The obtained results indicated that plants produced from seeds inoculated with nitrobine at the rate of 150% from the recommended dose gave the highest number of spikes/m², 1000 grains weight, grain yield/feddan, straw yield/feddan., carbohydrate % and protein% in grains in both seasons. - Application of nitrogen fertilizer at the rate of 70 kg N/feddan surpassed all the other treatments in all measured parameters in both seasons. - The interaction effect between biofertilizers and chemical nitrogen fertilizer rates was significant on all studied characters in both seasons. Wheat plants produced from grains inoculated by nitrobine at the rate of 150% and received nitrogen fertilizer at that rate of 70 kg N/feddan gave the highest values of studied traits in both seasons. Treatment having nitrobine at the rate of 150%