Two filed experiments were carried out at the Exp. Farm, Fac. of Agric.,Al-Azhar Univ., Nasr City, Cairo, Egypt, to study the effect of bio-fertilizers i.e. without added (Control), Microbein, Nitrobein and Cerialein and nitrogen fertilizer rates (0, 25, 50, 75 and 100 kg/fad.) and their interaction on yield and yield components of wheat Misr 1 cultivar during the two successive growing winter seasons of 2012/2013 and 2013/2014 seasons. The experimental design was split plot design with four replications. The main plot was devoted to biofertilizer treatments and the sup- plot contained nitrogen rates. Microbein bio-fertilizer recorded the highest yield and its component, except, spike length, no of grains/spike and 1000-grain weight which found with cerialein in both seasons.  On the other hand, the highest grain yield/fad recorded with the highest nitrogen level in both seasons. The interaction between bio-fertilizer and N rates proved to be significant for all studied traits. The highest grain yield/ha was found with the interaction between bio-fertilizers and 75 or 100 N/fad in the second season, which were no significant effect between them. According to these results, rate of 75 kg N/fad and all bio-fertilizer using variety Misr-1 could be recommended for favorable wheat production under the local environmental conditions of this study.