The present study was conducted to evaluate the ecological characteristics and the bioactive metabolic products of C. procera. Also, the crude methanol extract of the aerial parts of calotropis was examined for antioxidant and antimicrobial potentialilties. The scrubland dominated by C. procera represented an advanced stage of desert vegetation and comprised 33 species related to 14 families. Tamarix nilotica, Alhagi graecorum, Launea mucronata, Trichodesma africanum and Hyoscyamus muticus were the common associates. The major life-forms were therophytes, geophytes and chamaephytes. The assemblage of this community belongs to eleven floristics categories with predominance of Sahara-Sindian, Sahara-Arabian and Sudano-Zambezian. The flavonoids and polyphenols contents of the aerial parts of C. procera were 0.185 and 0.37 g/100g, respectively. Thirteen fatty acids were detected among them undecanoic, palmitic, lauric, myristic and linoleic acids. The Calotropis methanolic extract exhibited free radical scavenging activity with IC₅₀ value of 0.35 mg ml^-1. It is showed antimicrobial spectrum only against Bacillus subtilis.