This study was carried out in Plant Tissue Culture Laboratory of Faculty of Environ. Agric. Sci. El-Arish, North Sinai, Suez CanalUniversity during the period from April 2013 to August 2014.The objective of this study was to investigate the effect of growth regulators and some additives on Capparis spinosa L. micropropagation. The results indicated that the highest significant No. of axillary shoots/explant, axillary shoot length, No. of leaves/shoot and main shoot length (4.22, 3.40 cm, 23.04  and 4.77cm, respectively) were recorded when nodal explants were cultured on MS medium supplemented with0.5 mgl^-¹BA combined with 0.1 mgl^¹ NAA during establishment stage. While in multiplication stage BA at 0.5 mgl^-¹ combined with 0.1 mgl^¹ NAA and 1.0 mgl^-¹diphenyelurea gave the highest values of axillary shoot length, No. of leaves/shoot and main shoot length (8.88, 17.74 cm and 11.66 cm, respectively). Moreover, addition of BA at 0.5 mgl^-1combined with 0.1 mgl^-1 NAA and 2.0 mgl^-1phloroglucinol (PG) recorded the highest values of No. of axillary shoots/explant, axillary shoot length, No. of leaves/shoot and main shoot length (11.00, 12.08 cm, 20.04 and 14.86 cm, respectively). Addition of sucrose to the medium at 50gl^-1 combined with Benzyl adenine at 0.5 mgl^-1and NAA at 0.1 mgl^-1recorded the highest growth parameters compared with other sucrose concentrations. Shoots were rooted successfully (70% rooting percentage) by addition of 2.0 mgl^-1IBA combined with 1.0 gl^-1 activated charcoal to MS medium. Plantlets were successfully acclimatized (92% survival) when cultured in peat moss and vermiculite 1:1) v/v(