The present study assesses the morphological and anatomical features of Emex spinosa (L.) compd., in addition to Investigation of the nutritive value, the bioactive constituents, antioxidant and antimicrobial potential of this polygonaceous herb. The moisture content (6.4%), fiber content (20.5%), lipid content (1.1%), total carbohydrates content (41.5%) and crude protein content (19.05%) and the nutritional value (253.9 k cal./ 100g) of Emex spinosa were detected. Also, the methanolic extract of Emex aerial parts contained relatively higher concentrations of Polyphenols, flavonoids, Alkaloids, Tannins and Saponins than that of the water extract. The methanol extract showed high antioxidant activity in terms of 2,2-azino-bis (3-ethyl benzothiazoline-6-sulfonic acid (ABTS⁺) cation radical and reducing power assays in comparison with ascorbic acid. Both water and methanolic extracts of E. spinosa display antimicrobial activity against the bacterial strains, Bacillus subtilis and Erwinia Spp. and the pathogenic fungus Candida albicans.