Safflower seed yield is generally lower than that of other oil-seed crops. So for this reason, it is necessary to increase safflower yield. Information regarding the effect of growth regulators and its suitable concentration on growth and yield of safflower is very limited. Therefore, two field experiments were conducted at the Desert Experimental Station in Wadi El-Natroon, Fac. Agric., CairoUniv., during 2012/2013 and 2013/2014 seasons. Three growth regulators i.e., Melagrow, gibberellic acid, cytokininwere applied at three different concentrations viz., 20, 40 and 60 ppm, as well as, the control treatment of distilled water. The results revealed that all safflower traits were significantly affected by the tested growth regulators, except seed index and seed-oil %. Foliar application of the growth promoter Melagrow gave higher values for all studied traits compare with GA₃ and Cytokinin, except plant height and linoleic%. Increase concentration of growth regulators from 0 up to 60 ppm significantly increased petal yield plant^-1 by 48.3% and 37.1%, seed yield fed^-1 by 26.4% and 21.3%, as well as, oil yield fed^-1 by 29.4% and 27.5% in the first and second seasons, respectively. Quality traits, in terms of carthamin%, carthamidin%, oleic%, and oleic/linoleic ratio were significantly increased by increasing levels of growth regulators in both seasons. The highest values of such traits were obtained at 60 ppm level. The interaction effect between growth regulators and concentration on number of capitula plant^-1, seed weight plant^-1, petal yield plant^-1, seed and oil yields fed^-1 was significant in both seasons. Spraying Giza-1 cultivar with Melagrow at 40 or 60 ppmfour times was recommended for improving yields and its quality.